Sentence examples for for optimal staining from inspiring English sources

Appropriate concentrations of each antibody were determined by titration for optimal staining prior to experimental use.

All antibodies were titrated for optimal staining.

Dilutions were determined by standard titration methods for optimal staining (strongest signal with least background staining).

The MCM2 antibody, generated as described by Todorov et al. [ 9], and MIB-1 antibody were incubated for 60 and 30 minutes at room temperature and used at dilutions of 1 500 and 1 100, respectively, for optimal staining effect.

When considering live cell labeling, the performance status of the cell is the most critical issue for achieving optimal staining.

To expand the application spectrum to FFPE tissues, an extensive array of pretreatments was investigated for an optimal staining result.

For an optimal staining result on FFPE specimens, heat-induced AR was tested with acidic, as well as alkaline buffers and standard enzymatic digests (see Table 1).

For example, MB alone has been widely used for staining, 7– 10 whereas double staining with CV and MB has been reported useful for esophageal EC. 11 Although an ex vivo study assessed the optimal staining conditions for EC, 12 that study was carried out on a limited number of resected porcine colons.

Subsequent experiments were performed using the optimal staining conditions for each reagent.

The aim of the present study was to evaluate the optimal staining method for EC in the colon.

We first set out to establish the optimal staining conditions for pMHC tetramers and dextramers so that we could compare the optimal detection parameters of each reagent.