Exact(3)
This study builds upon previous work (Cheang et al, 2009) using a cut point for optimal determination of 'high' Ki-67 proliferation rate at 14% through correlation with the PAM50 classifier using RT PCR.
For optimal determination of MMP-9, precoating with F ab 2 fragments of goat-anti-mouse IgG-Fc (Jackson, West Grove, Pennsylvania, USA) in 0.1 M carbonate buffer (pH = 9.6) for at least 48 hours was done before coating of the capturing antibody.
For optimal determination of MMP1, MMP9, and TIMP-1, 96-well plates (Greiner M129A) were precoated with F ab 2 fragments of goat-anti-mouse IgG-Fc (Jackson, West Grove, PA, USA) in 0.1 M carbonate buffer (pH 9.6) for at least 48 hours before coating of the capture antibody.
Similar(5)
A procedure for the optimal determination of process synthesis, design and operation for both batch and continuous separation is presented.
This work discusses a sequential niching algorithm for multiple optimal determination.
An adjustment for low risk effects is not considered in the procedure of OECD guidelines, which is typically proposed only for the optimal determination of EC50.
A high specificity was found for Ki-67 and cyclin E, but sensitivity was higher for blastemal Ki-67 than for cyclin E. Optimal determination of these cutoffs needs a larger cohort of patients and a centralized immunohistochemical study to avoid technical variations.
The injection of samples into the GC system is an important and crucial step for the accurate and optimal determination of compounds with relatively high boiling points like PBDEs.
Related(2)
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