Sentence examples for for one rt from inspiring English sources

The RT reaction was done following manufacturer's instruction (Invitrogen, CA). 1 µg of total RNA and 50 ng of random hexamer were used for one RT reaction.

Quantitative RT-PCR was performed using the Rotor-Gene® SYBR® Green RT-PCR kit (Qiagen, Hilden, Germany) according to the relevant manufacturer's protocol for one step RT-PCR.

Equal amounts of total RNA were used for one step RT-PCR (QuantiTect Probe; Qiagen).

QuantiTect™SYBR®Green system (Quiagen cat#204243) was used according to the manufacturers instructions for one step RT-PCR in a total of 15μl reaction volumes including 0.5 μM each primer and 40ng RNA.

The analysis of SILV transcripts in differently pigmented skin and non-pigmented body tissues detected more than one RT-PCR fragment for several primer combinations.

Quantitative real-time RT-PCR was performed with an ABI Prism 7900 sequence detection system (Applied Biosystems, Foster City, CA, USA) and a QuantiTect Probe RT-PCR Kit (Qiagen, Chatsworth, CA, USA) for one-step RT-PCR.

Conditions for one-step RT-PCR were similar to those for multiplex one-step RT-PCR.

His order was for one "SMB 16.5 RT" (smallmouth bass, 16.5 inches, with a right-hand turn to the body).

Frozen tissue slices were thawed for one h at RT and hydrated with PBS.

Ovaries stabilized in RNA later for one wk at RT and two wks at 4°C were used for protein extraction.

The slides were washed again and incubated for one h at RT with 10 μg/mL anti-FLAG HRP-conjugated monoclonal antibody (Sigma).