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This can be achieved using 96-well or 384-well microplates so that each single well can be a site for one reaction.
For semi qRT-PCR, 1.5 μl of cDNA and 5 μl of primers (10 μM) were used for one reaction.
Each group has 4 biological replicates with 3 replicates for one reaction and each reaction was repeated twice.
Typically, data from four or five different time points were collected to analyze as a set for one reaction.
For one reaction 12.5 μL 2x SABiosciences RT qPCR Master Mix, 1 μL cDNA, and 11.5 μL RNase free water were used.
Initial allele ratio RiA/B being constant for one reaction tube, K2 would relatively be constant in only a few chosen cycles.
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For one-reaction systems, one stable steady state is born at a transcritical bifurcation.
In Lyon, experts at Dr. Dubernard's hospital, which is a leader in immunology, monitored her immuno-suppressive drugs and treated her for one rejection reaction.
For XR, one reaction was used for each coenzyme.
For any one reaction, the "occurrence" plotted on the vertical axis of the figure is the fraction of genotypes in this sample that contain the reaction.
On this level, evolution would be unconstrained for any one reaction, if the occurrence of the reaction in a metabolic network can vary independently of other reactions.
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