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Twenty-five sampled tissues for each organ were bulked into five pools (five sampled tissues for one pool), and each pool was used for DNA extraction with the Viral Gene-spinTM Viral DNA/RNA Extraction Kit (iNtRON Biotechnology).
The commissioners were going to tour the old and new Don Dale facilities and the Darwin watch house on Wednesday and media were not happy to find out there would be space for one pool camera from the ABC to attend and provide video to other organisations, but not for another journalist or photographer to ask questions or report on behalf of other media.
For the PBDE metabolites, there was not sufficient serum for duplicate analyses to be carried out for the children, except for one pool.
Tester cDNA was split into two equimolar aliquots to which different PCR primer adapter sequences were ligated (adapter A for one pool of cDNA and adapter B for the other); the primer sequences are described in the PCR-Select kit manual (Clontech).
Its new shared data plans, which allow customers to pay for one pool of data and share it across multiple smartphones, tablets and laptops, are helping it bring in more money from subscribers over all.
This trace illustrates an idealized average firing rate for one pool of LIP neurons under balanced reward conditions.
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However, most of these methods are based on substantial post-PCR processing, and for one or very few SNPs for one pooling sample as a time.
To provide measures of the consistency of differences between pooling approaches under repeated sampling, we plotted the prediction accuracies observed for one pooling approach against those observed for the other in the same 50 data sets.
This would argue for a general role of ARF5 in promoting vascular differentiation and for the existence of one pool of stem cells marked by PXY promoter activity and feeding both xylem and phloem production.
The dominant structural motif from one pool ("70A", for 70 random nucleotides, selected to bind Co A ) binds ATP in solution with a dissociation constant, Kd, of approximately 1 μM.
A population of legorgs was drawn at random from the gene pools, for practical reasons one pool for each gene, and constructed according to the morphogenetic rules.
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