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The first step is the creation of cross-section for observation under a microscope before other analysis.
Then the sections were stained with freshly dispensed diaminobenzidine solution (DAB) for observation under a microscope.
However, detailed examination of a mouse fetus in the past has involved thin sectioning for observation under a microscope.
For CD31/periodic acid Schiff (PAS) double staining, the sections were still incubated with 1% periodic acid for 15 min and Schiff reagent for observation under a microscope at 37°C between DAB staining and hematoxylin counterstaining.
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(iii) Smear Preparation for Observation under the Microscope.
Infiltrated leaf discs were collected 3 5 d later for observation under a confocal microscope.
Then, liver sections (5-mm thick) were stained with hematoxylin and eosin for observation under a light microscope.
The labeled filaments were then pelleted and captured on poly-L-Lysine coated glass slides for observation under a fluorescence microscope.
The samples were cleared in xylene and mounted in neutral gum for observation under a light microscope (type DX51, Olympus, Japan).
The thoracic pieces were transferred to 70% glycerol in PTw, and mounted on slides using SlowFade ® Gold (Invitrogen) for observation under a confocal microscope TCS-SP5 AOBS (Leica).
Finally, antibody binding was visualised using DAB as the chromogen and the sections mounted in Mowiol for observation under a Nikon 90i microscope.
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