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For nucleus staining Vectashield medium containing 4,6- diamino-2-phenylindole (DAPI Vector Laboratoriess) was used.
This is shown as green color for nucleus and red color for actin filaments.
Interobserver and intraobserver agreement for nucleus optical clearing were slight and moderate, respectively.
Apoptosis was assessed by staining with Hoechst 33342 for nucleus condensation as described in 'MATERIALS AND METHODS'.
Semitone mapping preserves the harmonic structure and may in turn improve music representation for Nucleus cochlear implants.
Advances in the delivery of these therapies using biomaterial scaffolds have also provided an avenue for nucleus pulposus replacement.
However, there has been limited work characterising the time-dependent mechanical behaviour of hydrogel scaffolds for nucleus pulposus tissue engineering.
This study investigates the potential of a carrageenan gel-infused polycaprolactone scaffold for nucleus pulposus tissue engineering.
The available literature relative to these criteria for nucleus and total disc replacement where relevant is also reviewed.
The decrease in particle size in modified ZnO could be due to possible coating during synthesis process where exposed bulky groups provide steric hindrances for nucleus agglomeration.
Fig. 5 a, b After cultured under stimulation and without electrical stimulation for 21 days, cells were immunostained with DAPI (blue) for nucleus, Tuj1 (red) and GFAP (green).
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