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B23 is important for nucleolar structure [ 43].
In contrast to fob1Δ, rad61Δ had less of a rescue effect for nucleolar structure.
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Effects of actD on nuclear structure were observed using immunofluorescence with nucleolar antibodies (Figure 4C) which showed typical disruption of nucleolar structure by drug treatment [46].
We believe the disruption of nucleolar structure by reduction of CTCF, PARP, or PARG to be similar.
In salivary glands obtained from CTCF35.2 homozygotes, we again observed dramatic vesiculation of nucleolar structure (Figure 3G), similar to the disruption we observed in S2 cell culture.
Perturbation of nucleolar structure and function leads to a response termed "nucleolar stress", characterized by the accumulation of the tumour suppressor protein p53 leading to induction of apoptosis, senescence or cell cycle arrest [ 18, 23, 42- 50].
This data implies p31Nesp1 is a potential nucleolar scaffold where the N-terminal SRs of the protein are critical for maintaining nucleolar structure in HDFs, which can be disrupted upon the overexpression of the N-terminally truncated p23Nesp1 or p12Nesp1 dominant negative isoforms.
B23, nucleolin, UBF and subunits of RNA pol I were found to be the argyrophilic proteins responsible for the silver-staining properties of nucleolar structures (Roussel et al. 1992; Roussel and Hernandez-Verdun 1994).
This model is supported by observations that inactivation of rDNA results in intermingling of nucleolar structures.
These results indicate that NPM is an essential protein not only for the formation of normal nucleolar structure, but also for the maintenance of regular nuclear shape in HeLa cells.
This review discusses current understanding of how the biochemical processes of ribosome biosynthesis relate to an observable nucleolar structure.
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