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Part of the supernatants was inoculated to shM2-MDCK for next passage of the virus culture, another part was subjected for plaque assay to determine if potential siRNA-resistant virus appeared.

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For L835 passage 36 was compared to primary tumor tissue, for L2975 passage 37 was used, and for L3252 passage 20 was compared to primary tumor tissue.

Indeed, BCG Danish appears to be continuing to evolve such that whereas cells with either duplication or triplication of the DU2-III region of the chromosome that contains sigH coexist in Danish 1331, only the triplicated form was found in a BCG Danish strain grown continuously for 1,513 passages [17].

They lack V(D J DNA rearrangements and vector DNA after expansion for 10 12 passages.

Proliferation of isolated cells was monitored for 15 25 passages, and a growth curve was calculated for each cell passage.

Over a period of 1 2 weeks, adherent, spindle-shaped cells were derived and subcultured weekly by trypsinization for ~10 15 passages.

Launched in 1936, she soon displayed scorching speed and wound up setting records well into the 1960s, including one for a 1964 passage from Los Angeles to Tahiti that stood for 30 years.

For example, early passage ASCs promote a proliferative response of allogeneic responder T cells in MLRs, whereas ASCs actually suppress the proliferative response beyond passage P1 [ 14].

Every 3 days the organisms were subcultured 1 10 in fresh medium and were kept in culture for a maximum of 20 25 passages.

Adherent cells were grown for 3-8 passages in culture (3 4 passages for GL26nu-1 & -2; 6 passages for GL26B6-1 & -2; 5-6 passages for GL26B6V-1 & -2; 8 passages for all others) RNA was extracted from cells grown to 80% confluence.

Nobody travels by foot anywhere, and nobody is responsible for the safe winter passage of the neighbors.

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