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Third to fifth passage cells were used for next experiments.
Therefore, a pH value of 5.0 was selected for next experiments.
Well-grown pure colonies were picked up and transferred on to mineral salt agar medium, and were stored at 4 °C until used for next experiments.
The complex was then lyophilized and used for next experiments [ 13].
APAP induced a dose-dependent mortality, and the dose of 3 g/kg was selected for next experiments.
We use 2 as θ for next experiments, since the processing time/evaluation ratio is reduced enough compared to the linear search and the nearest neighbors are close to the queries compared to top- k nn search.
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For SU5402 experiments, three or four beads were grafted into the anterior area opaca and the embryos grown in New culture for 1 hour.
Lipofectamine-3000 (Lipo3000) complexes were prepared according to manufacturer's protocol using the same plasmid DNA amounts used for 1,4C 1,4Bis GNP experiments and used to treat cells.
We downloaded (01/07/2005) A. thaliana gene expression levels for 1877 experiments from the NASCArrays database [ 81], using the bulk data download option.
Membranes were blocked using either TBS-Tween with 5% w/v skim milk powder (blocking buffer) for Ca2+-S100B Ca2+-S100Bs, or 1X Blockexperiments or, Canada) in TBS-Tween for apo-S1X0Blockeriments, followed by washing with TBS-Tween.
For summer, experiment with orange tones - a big catwalk trend.
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