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These derivatives were assayed for nematicidal activity.
Recently, a work by Lu et al. (2014) investigated 219 bacterial strains from cow dung for nematicidal activity against model nematode Caenorhabditis elegans and out of these, 17 strains killed more than 90%% of the tested nematode within 1 h.
Because of the principle differences between CBA (to determine IC50 for growth inhibition) and HTS methodologies (to determine LD50 for nematicidal activity), a direct comparison of the two methods in compound efficacies does not appear to be practical, nevertheless.
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Several mechanisms of nematicidal activity have been offered to explain how EPNs could have an effect on plant parasitic nematodes.
A brief investigation on the structure activity relationships (SAR) revealed that the targeted modification by a C7 hydroxyl was optimum compared with that of a C4 hydroxyl and that the coupling chain length was crucial for the nematicidal activity.
Their fusants were tested for their chitinase and nematicidal activity using bioassay and greenhouse experiments.
Thus, the extracts of F. vulgare and isolated biologically active compounds have been evaluated for their insecticidal, repellent, acaricidal, larvicidal, and nematicidal activity [ 115– 115].
This gene (plu2222) encodes a protein with homology to proteins listed as nematicidal proteins in GenBank but for which we can find no primary reference describing their nematicidal activity.
Protoplast fusion was a powerful technique in improving nematicidal activity.
This was for the first time that strains in the genera Proteus, Providencia and Staphylococcus from cow dung displayed nematicidal activity.
Chitinase production is an important factor in improving the nematicidal activity of such microorganisms.
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