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Saguaro provides conversion tools for Multiple Alignment Format (MAF), Variant Call Format (VCF), and multi-FASTA format of aligned genomes.
The necessary use of heuristics for multiple alignment means that for an arbitrary set of proteins, there is always a good chance that an alignment will contain errors.
This algorithm works especially well when adequate numbers of sequence homologs are available for multiple alignment.
The ClustalX program [33] and the TreeView program [34] were used respectively, for multiple alignment of homologous sequences and for construction of the phylogenetic trees.
Four alignment strategies used Clustal W [48] and SATé [21], and MrBayes [49] were applied in this study: the whole-ITS Clustal W. Clustal W is used for multiple alignment and MrBayes is used for phylogenetic inference.
Phylogenetic analyses were carried out with the "Phylogeny.fr" web server (http://www.phylogeny.fr), using Muscle for multiple alignment and GBlocks for alignment curation, and constructing the phylogenetic trees with PhyML [84], [85].
The situation is even more problematic for multiple alignment.
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Protein sequences were aligned using MegAlign (DNASTAR) for multiple alignments and EMBOSS http://www.ebi.ac.uk/Tools/emboss/align/index.html for pairwise alignments.
To test this, all IS elements with lengths >90% of the full sequence were aligned using the built-in Geneious 5.6.6 [ 37 ] algorithm for multiple alignments (default parameters).
In this thesis we develop new posterior decoding based methods for multiple alignments of discrete characters.
ClustalW [31] was used for multiple alignments with manual refinement.
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