Sentence examples for for more efficient expression from inspiring English sources
pGL4.20 was chosen as it is optimized for more efficient expression in mammalian cells, compared to previous generations of luciferase constructs, and contains fewer transcription factor consensus sequences such that background luciferase expression is reduced.
Some codons of this synthetic luciferase 2 have been optimized for more efficient expression in mammalian cells (GenBank AAV52869.1).
However, for the mouse CRD, more efficient expression was obtained by production of the CRD as inclusion bodies that were dissolved in guanidine hydrochloride and renatured by dilution and dialysis.
After correction has been made for the 2.1-fold more efficient expression of 4mtNELF-B (data not shown), in which case 4 ng of 4mtNELF-B in Figure 5H corresponds to 8.4 ng of wt NELF-B plasmid, 4mtNELF-B is seen to be 1.9 ± 0.5-fold (standard error of the mean; n = 23; P = 0.0003) less potent than wt NELF-B with different concentrations of dnCdk9.
The shorter oligonucleotides required (45 bp versus 64 bp for the shRNA construct) also makes this vector a more efficient expression vector for multiple siRNAs.
One clone designated BJ-ME (BHK-JEV-ME) that showed obviously more efficient expression of the E antigen was selected and maintained for further characterization and antigen production.
Thus, we used electroporation for more efficient gene expression instead of chemical transfections and increased the efficiency up to 65%.
It has been hypothesized that the higher copy numbers are required in free-living nematodes for more efficient regulation of gene expression and for responding better and more quickly to environmental factors such as temperature, nutrient availability, metal ions or pH [ 10].
The model was further improved by linking and expression as a single chain for more efficient structure determination studies, as well as incorporation into a membrane.
We also show that this siRNA expression cassette can easily be shuttled into a retroviral expression system and used to generate recombinant viruses for more efficient cell transduction.
