Sentence examples for for monitoring plasmid from inspiring English sources

For monitoring plasmid instability, the second is to be preferred.

The control plasmid for monitoring transfection efficiency is MT-RLuc [ 40]. miRNA plasmids were constructed by cloning a DNA segment containing the predicted primary transcript of each miRNA into the BamHI/EcoRI site of ActMSI [ 40].

Relocation into the nucleus of the yeast cytoplasmic linear plasmids was studied using a monitor plasmid pCLU1.

PIGQ-deficient CHO cells (10.2.1) (78) were transiently transfected with FLAG-tagged WT or mutant PIGQ cDNA, driven by an SRα promoter (pME FLAG-PIGQ) together with a luciferase expression plasmid for monitoring transfection efficiency.

Briefly, per transfection reaction, 0.1 μg reporter DNA and 2 ng PRL-SV40, a constitutively expressed renilla luciferase plasmid for monitoring transfection efficiency, were incubated with Fugene 6 transfection reagent (Roche Diagnostics).

The lambda promoters-GFP fusion plasmids [28] were used for monitoring the lambda infection process.

pCMVβ-galactosidase plasmid (Clontech, Palo Alto, CA, USA) was used for monitoring transfection efficiency.

The EGFP expression plasmid served as an internal control for monitoring transfection efficiency.

A pRL-Tk plasmid encoding Renilla luciferase was employed for monitoring transfection efficiency.

Plasmid Renilla luciferase (PRLSV40) was used as internal control for monitoring transfection efficiency.

Second, autophagic flux was traced by transfecting cells with an mRFP-GFP-LC3 plasmid according to updated guidelines for the use and interpretations of assays for monitoring autophagy.19 In cells labeled with mRFP-GFP-LC3, yellow puncta (mRFP and GFP signals) represent autophagosomes (APs), whereas red puncta (mRFP only) represent autolysosomes (ALs).