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a Sampling a garden gatepost for microscopy and analysis.
The hairs will be fixed, immunostained for γ-H2AX, and processed for microscopy and analysis.
Inductively coupled plasma mass spectrometry (ICP-MS) was carried out to quantify Pt using an Agilent 7500a ICP-MS (Centre for Microscopy and Analysis, Trinity College, Dublin, Ireland).
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Conditions for microscopy and data analysis were identical to those outlined in the legend to Figure 1.
We thank Lukas Neukomm for pLN178 and pLN180 plasmids, Gery Barmettler and Therese Bruggmann from the center for microscopy and image analysis at the University of Zurich for TEM sample preparation and Michel Labouesse at the IGBMC Strassbourg for the EM worm freezing protocol.
Cells were incubated in the dark for 15 minutes at room temperature, then centrifuged at 10,000 rpm and resuspended in 1X PBS for microscopy and FACS analysis.
All patients failing treatment, defined as reappearance of parasitemia after its initial complete clearance or failure of complete clearance within 28 days, were retreated whether they became symptomatic or not and after samples had been obtained for microscopy and PCR analysis.
JCS performed all cell conjugation experiments, preparation of samples for electron microscopy and analysis by electron microscopy.
Processing for electron microscopy and analysis were done according to the method of Dasgupta et al., 2010 [ 16].
WA) for assistance with cDNA library construction, Steve Mole and Leon Hodgson for help in plant culture, staff of the Centre for Microscopy, Characterisation and Analysis (CMCA) at UWA for assistance in microscopy and Lena Soo for her help analysing peptide sequences.
We also thank Erwin Wiegerinck (Department of Clinical Chemistry, Radboud University) for his assistance with hepcidin assays and Tracey Lee-Pullen (Centre for Microscopy, Characterisation and Analysis, University of Western Australia) for cytokine analyses.
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