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The slices are then turned into slides for microscopic study.
Older cytogenetic techniques involve placing cells in paraffin wax, slicing thin sections, and preparing them for microscopic study.
Convinced of the value of the microscope as a tool in all sciences, Sorby began to prepare thin sections of rocks (about 0.025 millimetre, or 1/1000 inch thick) for microscopic study in 1849.
The moulds were prepared in cold medium using epoxy-resin and hardener and were subsequently polished for microscopic study.
For microscopic study, a dimensionless function ξ(ca,cb) is proposed to evaluate the degree of mixing of particles on the scales of particle size.
After washing with water, the cells were counterstained with nuclear fast red for 5 min. The stained cells were dehydrated with gradient ethanol (70%, 95 % and 100), cleared with xylene and adhered onto glass slides for microscopic study.
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As only 8% of the total volume of the six biopsies selected for microscopic studies were in fact investigated in microscope, it is not surprising that the activity concentration differed with 28% comparing sphere counting in microscope with detector measurements on the same six biopsies.
The walls of lymph vessels are so flabby and collapsed that they often escape notice in specimens prepared for microscopic studies.
Plasmid pUCP19GFP (GFP+ Apr), which constitutively expresses gfp, was used to label the strains for microscopic studies.
Moreover, N. benthamiana is also emerging as an excellent system for microscopic studies of infections caused by filamentous plant pathogens, such as Phytophthora spp., given that tissues can be mounted and analysed without prior treatment [24], [25].
For microscopic studies, BAEC were cultured to confluence on 0.5%.
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