Exact(3)
Thus, the observation for microsatellites of low FST in African populations can be attributed to high within-population genetic diversities.
Assuming a mutation rate for microsatellites of 2.96 × 10-3, estimates of effective population size indicate that the ancestral population was significantly larger (3,924 individuals, 90% HPD, 2,598-19,005) then the populations after divergence.
We examined repeat array length and purity (number of mismatches with respect to the consensus repeated motif) for microsatellites of at least six copies in hotspots and other regions of the yeast genome.
Similar(57)
A study to mine SSRs in silico from 22298 EST sequences of eucalypts revealed that primers could be designed for 1244 microsatellites, of which 182 were selected for characterization based on polymorphism status among species (Grattapaglia et al. [2014]).
We tried to amplify homoeologous microsatellites from Triticum urartu (2n=2x=14, AuAu) and Triticum monococcum (2n=2x=14, AmAm ) using 54 sets of PCR primers originally designed for the microsatellites of the A genome of common wheat, and found that 26 primer sets amplified microsatellite products in all seven accessions of T. urartu and all three accessions of T. monococcum.
This central role for microsatellite analysis of colorectal cancers involves a growing trend toward "universal testing" for evidence of MSI, whether by PCR methods or by immunohistochemistry (IHC).
Second, we demonstrate a directionality bias in somatic human cells that favors expansion mutations for mature dinucleotide microsatellites of 13−20 units.
This strongly suggests that repeat length does affect the degree of polymorphism for microsatellites.
In the case of microsatellites, some simulation replicates demonstrated dramatic departures from the mean value of D for microsatellites.
Our results give further insights into the mode of microsatellite evolution, and support the assumption of increased slippage rate with increased microsatellite length and a stabilizing effect of interrupting motifs for microsatellite regions consisting of perfect repeats.
The most conventional procedure for the isolation of microsatellite markers, i.e., enrichment of genomic DNA for microsatellite motifs cloning, screening of the resulting library and sequencing of the positive clones [ 4], is challenging, time-consuming and costly.
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