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To allow for maximal sequence alignment, dashes were inserted in the sequence.
Both SNP and PCR-RFLP solutions computed by the SPR Opt software include multiple loci when necessary for maximal sequence discrimination.
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To avoid cross-hybridization between these probes and targets, we arranged for the maximal sequence overlap among these probes to be less than 6 nucleotides.
These data suggest that for applications in which maximal sequence diversity is essential, such as epitope mapping or novel receptor identification, combinatorial peptide libraries should be constructed using codon-corrected trinucleotide cassettes within vector host systems designed to minimize morphogenesis-related censorship.
Thereafter, we saved the found maximal sequence for the given gene, its coordinates in the given gene and periodicity matrix M, which shows the type of TP found.
The CATHSCOP set contains lists of templates of consistently classified domains for 4859 target domains with pairwise maximal sequence similarities of 50%.
Loops were modeled by scanning a nonredundant subset of the PDB (≫8000 structures) for fragments with matching anchor points, a minimal number of bumps, and maximal sequence similarity.
For adaptor trimming cutadapt (version 1.2.1) was used with the following parameters: minimal sequence length after adaptor removal was 16, maximal sequence length was 28 [ 65].
We conclude that phylogenetic informativeness profiles provide advantageous guidance for phylogenetic projects in the selection and prioritization of loci to sequence for maximal return on effort and investment.
Of the elements tested here, the BYDV BTE and UTR sequences appear to be the most effective cis-acting sequences for maximal translation in wheat germ extract, while the TNV BTE and UTRs provide most efficient cap-independent translation in tobacco cells.
Both BACs were needed for maximal representation of upstream sequences both proximal and distal to appb transcription start site in enhancer-trap BACs (see Methods, and reference [ 14]).
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