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High quality (>100) homozygous SNPs with coverage higher than 15× were kept for marker development.
We thank C. Pikaard, A. Herr and D. Baulcombe for discussions and preprints and the Monsanto Company for marker information.
We have used a bioinformatics approach to search for marker sequences contained in open reading frames.
Procedures for marker imaged processing, and missing marker recovery are also surveyed.
Differentiation was assessed by alkaline phosphatase (ALP) activity, mineralization, and mRNA expression for marker genes.
In total, a set of 97 primer pairs was successfully designed for marker development and validation.
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Markel, P. et al. Theoretical and empirical issues for marker-assisted breeding of congenic mouse strains.
Our present efforts focus on use of these maps for marker-assisted selection.
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Cloning ZDS gene and developing functional markers are important for marker-assisted selection in wheat breeding.
Programmable microfluidic genotyping of plant DNA samples for marker-assisted selection.
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