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Although some organic matter may be decomposed at this temperature, complete drying is important for long term experiments and for extrapolating results obtained toward industrial applications.
For long term experiments the number of adherent VPC was determined after staining with PE-conjugated antibodies against CD105 (BD Biosciences) and CD144 (BD Biosciences) at day 14 after infection.
For long term experiments, control and senescent media were aliquoted and frozen in -80°C until use.
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It is also difficult to maintain stability for long-term experiments and projects.
In this paper, we compare identity preserving 3D tracking approaches for long-term experiments considering biological usability.
However, difficulties in manipulating the droplet environment and monitoring encapsulated bacterial population for long-term experiments limit its utilization.
Experiments are graded as class I (European category: "non-recovery") for short-term experiments, class II (European category: "moderate") for long-term experiments in non-septic control animals and class III (European category: "severe") for long-term experiments in septic animals [16].
Our Raman extension is suitable for long-term experiments lasting several days, exploiting the typical operational parameter space of the PPMS. Figure 2 Detailed view of the optical set-up.
Data analysis for Long-Term Experiments (LTEs) with cropping systems requires some careful thinking, especially for the most complex designs, characterised by rotations with different durations and/or a different number of test-crops per rotation cycle.
The ultramicroelectrode response was stable under harsh conditions of 1 mM H2O2 during the first hour, while the response decay during the second hour was less than 4% providing sensor suitability for long-term experiments.
For long-term experiments the cells were measured in the described DMEM medium.
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