Anti-fetal hemoglobin primary antibody was used at 1 1000 dilution (ab156584, Abcam, RSA), for loading control, 1 5000 dilution of anti-p38 (BioRad, USA) and 1 5000 dilution of goat anti-rabbit IgM horse radish peroxidase (HRP) conjugate secondary antibody (BioRad, USA) were used.
Actin was used for loading control.
Protein detection for actin was used for loading control.
All membranes were incubated with anti-α-tubulin (1∶500) for loading control.
Mouse anti-beta-actin antibodies (Abcam, Cambridge, MA, USA) were used for loading control.
The GAPDH-specific primers were used for loading control (IDT technologies, Corallevielle, IA).
For loading control in Western blots anti-protein disulphide isomerase antibody (aPDI) was used (ab2792, Abcam).
The β-actin specific primers were used for loading control (IDT technologies, Corallevielle, IA).
For loading control, mouse actin-specific antibodies (Sigma Aldrich) were used.
For loading control, the same blot was incubated with an anti-tubulin antibody.
Blots for loading control were stripped subsequently using ReStore® Western Blot stripping buffer (Thermo Fisher Scientific) then re-probed for GAPDH (Millipore, Billerica, MA, USA).
