An automatic data logger unit had been used in order to record and store data during the test for load cells, strain gauges and the LVDTs.
For calcein loading, cells were incubated for 30 min with 5 lm calcein-AM at 37 °C, centrifuged and the pellet was resuspended in fresh HBS.
The sarcolemmal membrane of the ventricular myocytes was visualized by loading cells for 10 min with the lipophilic fluorescent indicator di-8-ANNEPS (5 μM; Molecular Probes).
Isolated cells were loaded with 4 μM fura 2-AM at room temperature for 15 min. After loading, cells were perfused with Na+-HEPES solution in the absence or presence of the experimental agents.
For loading the cells, substrate was diluted directly in the medium for a final concentration of 1.5 µM and incubated for 60 min at room temperature and then replaced with fresh medium.
This transporter is also important for loading CPE cells with HCO3-.
For loading experiments, cells were seeded on macroporous Optimaix® collagen-I scaffolds (Matricel) at a density of 3.2×105 cells/scaffold (cylindrical shape of the scaffold; diameter 5 mm, height 3 mm).
It will be demonstrated that the controller design for EMC load cells can be based on the derived parameters.
We then performed ex vivo tests on longitudinally cut aortas coupled to a loading cell for force measurements with the Extroducer, scaffolded by iron tubing (fig. 2a).
The complete calibration details for the custom built load cells for normal and friction force measurements are furnished.
