Values for killed controls were subtracted from those for live samples.
However, to prepare a sample to be imaged by an electron microscope is a rigorous process that does not allow for imaging of live samples [ 42].
The penetration of confocal images for 3D segmentation of live samples could be improved by using multi-photon confocal microscopy.
Mathematically, "artificial samples" represent a subset of "fake samples", because the set "fake samples" may also include modifications of live samples [ 9, 11], for example, "artificial samples" are fingerprints produced from a mold, but the set "fake samples" contains also injured or otherwise modified live fingers and biometric samples.
For his Looking Ocean EP, released last year on Scion A/V, Daedelus tapped his close friend, the brilliant pianist Austin Peralta, whose work spanned across many collaborations and genres, before sadly passing last November at the age of 22. Daedelus is known for his live sample-manipulation on the Monome, which he performs with the same ease as Peralta on the keys.
By incorporating an additional opening in the cavity, it could act as east access inlet for samples, as well as an entrance for live aquatic samples in the future.
For confocal imaging, live samples were inverted and mounted on an inverted Olympus IX 81 microscope with confocal scanner unit (CSU-W1, Yokogawa, Japan) and imaged with a ×60/NA 1.42 oil U PLAN S APO objective.
For live imaging, samples were loaded onto a stage heated to 37°C by a fan and antibody staining was not used.
Image acquisition is performed by scanning the focal volume of the laser, which allows for nondestructive (and therefore potentially live sample) imaging in three dimensions at submicrometer-scale spatial resolution.
During their live shows of the 1990s, the Orb performed using digital audio tape machines optimised for live mixing and sampling before switching to laptops and digital media.
