The first model is inverse splicing, in which the downstream exon turns around to become proximal to the upstream exon and ready for splicing, resulting in a circular and exon-skipped linear transcript.
For example, the promoter of the Sry circRNA transcript differs from that of canonical linear transcript.
The more credible way to recognize circRNA for a specific gene is using PCR with out-facing primer pair (located near the back-splicing sites on the corresponding linear transcript), though false-positive cannot be excluded because of various reasons.
Second-strand cDNA synthesis was followed by in vitro transcription for linear amplification of each transcript and incorporation of biotinylated CTP and UTP.
Adjustments of mitochondria-associated transcript and protein expression were probed and assessed for linear relationships to cellular and functional variables of oxygen metabolism.
The lyrics don't settle for linear explanations.
For most transcripts linear amplification is robust and reproducible.
For this viral transcript the linear dynamic range spanned from ten copies to one million copies (highest tested).
As shown in Figure 1, for the transcript data a linear regression of expression levels against growth was made for each gene in each nutrient limitation in turn, and the slope of the regression was determined.
We had also fitted linear fixed effect models for each transcript in each brain region.
