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For light microscopy (LM), pollen was preserved in silicone oil.
Photomontage and lettering was done as for light microscopy.
Tissue was prepared and fixed as for light microscopy.
Thin sections were then routinely prepared for light microscopy analysis.
Ten µm-embedded frozen sections were stained with hematoxylin and eosine for light microscopy histology examination.
For light microscopy, the cells were fixed with Bouin's fixative and stained with Giemsa (Merck).
For light microscopy, serial paraffin sections, 3 µm in thickness, were produced at three levels.
For light microscopy, 7µm serial paraffin sections were cut and dried at 37°C overnight.
Sections from both septal and temporal hippocampus were prepared for light microscopy.
For light microscopy, 3 µM paraffin sections were stained with Periodic acid-Schiff's (PAS).
They were cover-slipped with DPX for light microscopy at ×40 magnification.
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