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The light harvesting complex protein Lhcb1, which is essential for light electron transport, is significantly regulated during bud release [ 11, 63, 66].
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QDs are inherently electron dense and therefore ideal cellular markers for correlative light electron microscopy (CLEM).
For correlative light electron microscopy (CLEM), samples were initially fixed in 4 % paraformaldehyde (PFA)/0.4%% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to an equal volume of zebrafish embryo water for 10 min, followed by post-fixation in 2 %PFA/0.2 % glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) overnight at 4 °C.
Eyes were processed for RNA extraction or light and electron microscopy analysis, and optic nerves were processed for light and electron microscopy as described (Supplementary Text).
The production of 'cell cortices' for light and electron microscopy.
Specimens were processed for light and electron microscopy.
Myocardial biopsy samples were taken for light and electron microscopy.
Six days after treatment, the goats were hemicastrated and the epididymis was isolated and processed for light and electron microscopy.
Knott, G.W., Holtmaat, A., Trachtenberg, J.T., Svoboda, K. & Welker, E. A protocol for preparing GFP-labeled neurons previously imaged in vivo and in slice preparations for light and electron microscopic analysis.
The spinal cord at L1, posterior and anterior roots and cauda equina were excised 5 days later, sectioned, processed, and prepared for light and electron microscopic examinations.
The thyroid glands were immediately removed from animals at the time of sacrifice, and the tissues were processed for light and electron microscopic examination.
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