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With the LCV techniques samples can be treated with ETDA and preserved for latter analysis.
The first and last counselling session will be sound-recorded for latter analysis to ensure that MI had been applied correctly.
The video images were stored in a computer hard disk for latter analysis using an appropriate software APAS (Ariel Performance Analysis System, Ariel Dynamics, San Diego, USA).
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One limitation of the two-step procedure used for the latter analysis is that for chaotic models the backward-forward solution of the ODE is ill-conditioned [ 23].
For the latter analysis, we obtained residence for each subject and each year from the Registered Persons Database (Toronto, ON, CA) for the period 1996 2010, and derived annual estimates of PM2.5 exposure by assigning the 6-year mean concentration of PM2.5 at each annual postal code.
For the latter analysis, we assigned the effective population size for mtDNA as four times less than for the S7 intron and the mutation rate was set four times greater for the cyt b gene.
For the latter analysis, the list of human-mouse orthologs was obtained by concatenating Affymetrix (http://www.affymetrix.com) and Ensembl Biomart (http://www.ensembl.org/biomart/martview/) annotation tables and including only one-to-one human-mouse orthologs.
The reasoning for the latter analysis is discussed in the Results.
For the latter analysis, socio-demographic variables found to be related to hospital admission were included as fixed covariates.
The genome-wide significant thresholds (P < 0.05) for this latter analysis were calculated from 2,000 permutation tests.
For the latter analysis, we integrated data with a recently generated tissue-specific dataset from FlyAtlas [ 25].
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