The pellet was then processed directly for RNA extraction or stored at −80°C for later processing.
One possible mechanism for cardiac phenotypic heterogeneity in Cited2 deficiency is that not only is it required for the earlier process of left right patterning but also independently for the later processes of atrioventricular septation, outflow tract, and aortic arch development.
For the later process, the pre-exponential factor in the ionic transport equation has been obtained from experiments involving sharp changes in the reanodization current.
Receiving nodes, within the sender's transmission range, create or update their neighbors list and use the information in the beacon packets for later routing processes.
There will be more concrete SEAs for later decision processes under the framework of the GWDS.
Although we and others largely focused on this traditional efficacy window, morpholino-based screening has also been effective at identifying novel loci critical for later developmental processes (e.g. [ 7]).
Furthermore, it has been showed that Dazl, the murine homologous of DAZ, plays a role in the transport of specific mRNAs via a dynein motor complex, and that the Dazl-bound mRNAs is stored at specific sites to be available and used for later developmental processes [ 19].
The construction process is the same as the CFFP-tree algorithm except more information are kept for later mining process to discover the completely fuzzy frequent itemsets.
Approximately 24 h after transfection, cells were transferred both onto coverslips and onto a well of a six-well plate and, 24 h later, processed for internalization assays and for protein expression, respectively.
Springer et al. [15] for instance, divides the signal into a sequence of segments which are classified and later processed for a precise localization.
The cultured cells were later processed for transfection experiments and western blot analysis.
