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The cells were firstly incubated with 7.5 μg/mL Dil-acLDL (Molecular Probes, USA) at 37°C for 4 h and later fixed with 4% paraformaldehyde for 10 minutes (min).
For this purpose, cells were first incubated with 10 μg/ml DiI-ac-LDL at 37°C for 1 h and later fixed with 2% formaldehyde for 10 min, followed by incubation with BS-1 lectin at 37°C for 1 h.
Cells were first incubated with 10 μg/ml DiI-ac-LDL at 37°C for 1 hour and later fixed with 2% formaldehyde for 10 minutes, followed by incubation with UE lectin at 37°C for 1 hour.
(a) Nef-VC and MHC-I-VN-Flag were transfected into HeLa cells, and 24 hours later fixed and immunostained for TGN46.
Ms. Kelly's bill included an erroneous (later fixed) $314 charge for an, um, male incontinence clamp.
For the study of recombinant Crym, cultured cells were seeded on glass coverslips, and transfected with recombinant Crym-HA vector or equivalent amount of empty vector for HEK293T cells, and 48 h later fixed with cold-ice methanol and EGTA.
Lungs were collected 4 hours later, fixed in 4% PFA overnight and paraffin-embedded for IHC.
A dangler (later fixed).
(This was later fixed).
Make it "lead" (later fixed).
This was later fixed online.
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