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When species determination was not possible by sight, bumble bees were caught with a net and either determined to species on site or brought to the laboratory for later determination.
The court having considered the affidavits, we conclude only that the District Court did not err by refusing to consider them; we leave for later determination by that court the question of the admissibility of testimony by Dr. Loube and Dr. Dingus under the Montana Rules of Evidence.
Dried shoot material was milled for later determination of total N and P concentration.
A digital photograph was taken from one side of the head from each parent kestrel for later determination of coloration of the cere.
At the end of each experiment, hearts were rapidly freeze-clamped with tongs cooled in liquid nitrogen and stored frozen at −80°C for later determination of enzyme activity.
Before sacrifice, venous blood was drawn for blood culture and serum samples (≥500 µl) were stored at −20°C for later determination of anti-PVL antibody and C-reactive protein titers.
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Samples were then frozen for later determinations of symbiont concentration using an inverted microscope (Leica, Wetzlar, Germany) and the Histolab 5.2.3 image analysis software (Microvision, Every, France), according to Rodolfo-Metalpa et al.77.77
Serum was separated and kept frozen at -70°C for later determinations of SAA, Hp and LBP.
At delivery, umbilical cord blood was collected for the later determination of serum ionized magnesium levels.
Inter-batch coefficient of variation was 8.2% A whole blood aliquot was stored at −80°C for the later determination of MTHFR genotype.
Set one was used for later genotype determination through microsatellites and flow cytometry as described above.
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