However, for large read lengths (400 nt) the performance of the baseline version largely decreases, whereas the improved versions clearly outperform the baseline version.
The read count error equals 5√N/N, which decreases as the read count increases and is negligible for large read counts.
CREST performs poorly in terms of recall for a read length of 50 bp, but then improves for larger read lengths (Additional file 6: Figure S6).
The latest PacBio RS II SMRT cells are designed to select for larger read-lengths when long insert libraries (10 to 20 kb) are being prepared, however, preferential loading of smaller fragments can still occur and this limits sequence output.
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For round #2 two different nested primers were used to yield slightly different size PCR products so bands resulting from specific priming could be easily distinguished Sequences obtained from PCR and bulk sequencing in Brassicaceae species were inspected for large intact reading frames.
For history writ large, read politics writ small.
It is possible to observe that there is some performance degradation at the end of the disk (the 37 40 GB region), mainly for large sequential read accesses.
It is the software package we developed previously for large-scale read mapping.
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