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The preparation method is simple, highly efficient, energy-saving, environmentally friendly, and has been demonstrated to be effective for large preparations of cordycepin from waste with low equipment and operating costs.
Plasmid isolation was performed using the cetyl-trimethyl-ammonium bromide method (CTAB) [ 42] for small preparations, and the JetStar kit (Genomed GmbH) for large preparations.
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The CCE method has high extraction efficiency, uses a minimum volume of solvent and can be used for both quantitative analysis and large preparations of cordycepin from waste.
Interestingly, we also show that in these conditions, DNA concentration can be maintained in the 1 mg/L range, thereby limiting the need for large DNA preparations.
Plaque isolates were purified three times and expanded for large scale preparations in Vero cells.
For large VEGFf preparations, the samples (NE and mock treated) were dialyzed (10-kDa molecular weight cut off, Slide-A-Lyzer; Pierce, Rockford, IL) against PBS at 4°C to remove DFP.
For large scale preparation of the recombinant protein, 1 L of bacterial culture was induced under the optimum condition.
Although the antimicrobial peptides were produced in NB for large scale preparation, the influence of different carbon and nitrogen sources on peptides production was tested using minimal medium.
Among various techniques, the sol gel approach is advantageous due to its low cost, amenable for large area preparation, and easy to handle properties, out of which the spin-coating and dip-coating techniques are widely used.
Clones that demonstrated recombinant expression as determined by SDS-PAGE analysis were further cultured for large scale preparation.
For large scale preparation of anti-HBV HD Ads, 2 liter cultures of 116 cells were coinfected with helper virus and anti-HBV HD Ad lysates.
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