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RT-PCR was carried out using the Advantage RT-for-PCR kit from BD Biosciences Clontech.
Primer sequences for KIT were designed from the KIT mRNA and genomic sequence (Ensembl gene ID=ENSG00000157404), using the Primer3 software (http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi).
Proteins were visualized using the SilverSNAP Stain for Mass Spectrometry kit from Pierce (Rockford, IL, USA) for extracellular proteins and Coomassie Brilliant Blue for intracellular proteins.
Perhaps the same could be said for the Socceroos kit from the early nineties – reviled in its time but hailed by football hipsters today.
At the last minute South had to scramble around for a new kit from a Brazilian supplier, and naturally this didn't go down well with their original kit supplier, Puma.
Mononuclear cells were then isolated and underwent immunomagnetic negative selection for CD14+CD16- monocytes (kit from Invitrogen) according to the manufacturer's instructions.
Oligo-dT primer-directed cDNA was synthesized using the Super-Script III First-Strand Synthesis System for RT-PCR kit from Invitrogen.
ELISA for mouse MIF was done under the instruction of manual for human MIF ELISA kit from R&D Systems.
For example, you can buy a starter kit from GHI Electronics for $249.95 at the end of September.
My mother helped fix it with a little of her "only your hair dresser knows for sure" home coloring kit from Clairol, and it gradually grew out.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com