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Mice were killed 4 weeks later for kidney examination.
For analysis of local invasion, the equivalent of 10 cells in 10 μl was injected and the mice were killed 4 weeks later for kidney examination.
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All ultrasonographic examinations were performed by a single expert operator using a Philips CX50 Echocardiography (Philips Healthcare, Eindhoven, Netherlands) with a S5-1 Sector Array transducer for kidney's examination with the patient in supine position.
This has advantages for patients getting a routine scan, where additional examinations for kidney function estimation could be avoided.
Kidney histological examination: The kidneys were collected at the end of experiment.
The consequences for kidney function were detrimental.
For routine histology examination, kidney and liver paraffin sections were subjected to periodic acid-Schiff (PAS) and hematoxylin and eosin staining, respectively.
For histological examination, kidneys bearing islets were harvested and fixed in 4% formaldehyde for 24 h and embedded in paraffin.
Kidney histology examination was by light microscopy only.
Kidney ultrasound examination was normal.
Then, all mice were sacrificed and collected their heart, liver, spleen, lung, and kidney organs for histological examination with standard techniques at day 16.
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