While there has been considerably less research into adult ADHD, the behavioral evidence for inhibitory deficits appears robust, with some support for its specificity compared with other functions [Bekker et al., 2005; Boonstra et al., 2010].
SOCS2 mRNA data were further corroborated by Western blot analysis with an antibody evaluated for its specificity by experiments with cells expressing recombinant SOCS2 protein (see Methods section).
From the hypothetical model of Asp-Ala bound YePEPT (Fig. 4), it appears likely that Lys314 is responsible for its specificity for dipeptides with negatively charged amino acids at the R1 position.
RADPRE is a fairly good choice for its simplicity and specificity with an estimated FDR of 1%.
In addition, G-quadruplex binding activity of TMPyP4 tetrachloride was also quantitatively compared between two types of human telomeric G-quadruplexes, i.e., DNA and its RNA anologue, for a purpose of exploring its binding specificity with nucleic acid G-quadruplexes.
For the antibodies with blocking peptides available, the antibodies (ERα, cyclin A, cyclin E) were evaluated for its binding specificity by incubation with 5 fold of blocking peptides before applying to cells for primary antibody incubation.
This commercial enzyme preparation contains the exopeptidase DPPIV, and is also widely used as a debittering agent in foods and dietary supplements due to its specificity for peptides with N-terminal X-P sequences.
Table 1 presents commonly used criteria for ECE with its specificity and sensitivity.
By virtue of its specificity for interfering with the first round of transcription initiation, this metal cluster compound serves as a useful tool for studying the role of TFIID in both transcription initiation and reinitiation.
