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The following additional information is available in the online version of this article – List of taxa used in this study, along with the GenBank accession numbers for ITS sequences and its references.
On the contrary, the insufficient database for ITS sequences may also be one of the reasons for such low similarity values (Zachow et al. [2009]; Anderson et al. [2003]).
Eighteen recently collected specimens of Geoglossum, Trichoglossum, and Thuemenidium from New Zealand, China, and Canada were sampled for ITS sequences in this study.
Categorical comparisons for ITS sequences also indicated that faster rates of substitution were correlated with annual habit.
Reports of multiple nrDNA haplotypes within individuals are becoming increasingly common [e.g. [ 43- 51]] suggesting that complete concerted evolution should not always be assumed for ITS sequences.
In addition, there was also significant differentiation for ITS sequences among isolates in Nagqu versus Qilian; Nyingchi versus Qilian; and Garze versus Yushu (Table 2).
Similar(47)
For ITS sequencing, all 28 isolates were first identified as C. guilliermondii, showing similarity between 98.34% and 100%.
For ITS sequencing, the presence of "double peaks" at polymorphic sites in the chromatogram was manually checked.
Eleven isolates, representative of each of 3 ITS-RFLP patterns obtained, were selected for ITS sequencing: ITS-RFLP profile A (S. prolificans, WM 06.378, WM 06.440, and WM 06.393), ITS-RFLP profile B (S. apiospermum, WM 06.389, WM 06.471, and WM 06.497), and ITS-RFLP profile C (S. aurantiacum, WM 06.388, WM 06.482, WM 06.495, WM 06.496, and WM 06.498).
The circuit has also been admired by fans and drivers alike for its sequence of fast corners, which includes the 200mph turn known as 130R.
IPI also maps to a growing set of qualified Gene Ontology (GO) terms for its sequence entries.
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