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Conversely, Hsp90 represses gene expression from Hsf1-dependent promoters [ 44], establishing a negative feedback loop for its own expression.
Since a TF should not necessarily be required for its own expression (positive feedback), in the case of JUND (and also NFKB1) the AND connection was changed to OR.
In contrast, analysis of met LacZ expression confirms that met is required for its own expression in the C7-C8 brachial pool, signalling via either PI3K or Src being equally efficient to ensure establishment of met expression domain.
To assess whether the specification and development of MC, ADF and PHA were affected by the ceh-19 deletion and also whether CEH-19 is required for its own expression in these neurons, their morphology was examined in the ceh-19 tm452) ceh-19 tm452kgroundeletionceh-19backgroundp fusings.
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nkx6.1 being dependent on Notch signaling for maintaining its own expression, this prompted us to compare the location of the Notch-responsive cells and the Nkx6.1+ cells using the Tg(Tp1 VenusPest) [ 16] or Tg(Tp1:eGFP) [ 12] lines in which fluorescent markers are under the control of Notch-responsive elements (Tp1).
In such a case, a mutation in the gene itself might be responsible for variability in its own expression at the mRNA level.
For instance, TGF- β enhances its own expression [ 23] and that of PDGF family proteins [ 23, 24].
This creates a positive feedback loop for FGF10, as FGF10 now enhances its own expression.
In other words, the PER/CRY complex inhibits its own expression, allowing for reactivation of BMAL1/CLOCK leading to rhythmic expression with a periodicity of 24 hours.
To evaluate the effect of combing two cell types on the gene expression of relevant genes, the change for each culture group relative to its own expression on day 1 (as different culture groups showed significant differences in baseline gene expression on day 1) was statistically assessed using the parameter ΔΔ Ct.
CDX2 regulates its own endogenous expression in different cellular contexts, initiating its own expression.
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