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Iron stained bone marrow slides were graded according to Gale's criteria for iron content in stroma, macrophages and red cell precursors [11].
In this context, HIMT assemblage from Classe, subdivided into the three subgroups (CL1a, CL1b and CL1c which differ mainly for iron content) is quite coherent with the coeval Mediterranean and European HIMT glass.
The samples were then allowed to cool at room temperature for 2 min, vortex mixed and centrifuged at 10 000×g for 10 min at room temperature [89], [90] and assayed for iron content.
The 2% FCS was analysed for iron content and showed to be 0.35 μM.
Urine was not collected or analyzed for iron content, because iron excretion in urine was assumed to be negligible.
For iron content measurement, cells were suspended in 10 mL 1 M HCl solutions for at least 24 hours.
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For iron contents above 25% the formation of α-Fe2O3 was detected, pointing out the formation of the isolated oxides.
The Fe isotope was used for the quantification of iron content in samples.
To overcome the problems associated with spectrophotometric measurements, a calibration curve for the estimation of iron content was prepared by the combination of two analytical methods.
As mentioned before, employment of spectrophotometric measurements as a simple and rapid method for the estimation of iron content in the starting material as well as in the processed samples might not yield reliable and accurate results.
Three-dimensional (3D) SWI has been proven superior to T2* and other existing magnetic resonance imaging (MRI) techniques for the detection of iron content and hemorrhage in brain [ 8, 9].
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