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Our results show that pBLIC vector retains the high transduction efficiency of the original pBABE while eliminating the requirement for checking individual cDNA inserts for internal restriction sites.
Further the LIC retroviral system can greatly facilitate high-throughput cloning processes such as those used for the creation and verification of retroviral gene overexpression libraries where screening individual inserts for internal restriction enzyme sites is impractical.
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Using Red/ET recombination-mediated mutagenesis for the targeted design, the work can be expedited and focused, in principal, on any sequence within the viral genome and is not dependent on the use of internal restriction sites.
This innovative program was intended to eliminate extended duty hours for internal medicine senior residents and to address some of the identified consequences of duty hour restrictions on residents' wellness, health care delivery and medical education experience.
But his analysis was for internal consumption.
Institutions use the results for internal diagnostics.
Smuggled cellphones were used for internal communications.
Rpl19 expression was used for internal normalization.
The author provided data for internal consistency.
No correction for internal weights was applied.
Reporting for internal and external clients.
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