The absorption intensity reaches to be as high as 65% for internal reflection and s polarization, around the blue wavelength for air/MoS2/SiO2 stack.
For internal reflection geometry (Kretschmann attenuated total-reflection geometry), half-cylindrical, half-spherical, or trapezoidal prisms are commonly used.
Highly refractive materials, for example Si, Ge, and ZnSe, are suitable for internal reflection optical geometry (Fig. 1a, (ii)) and CaF2 and BaF2 are more suitable for transmission geometry (Fig. 1a (i)).
In practice, the reflector is replaced by a plane for total internal reflection with correction for Goos–Hanchen shift.
The inner surfaces of the 3-D mask were coated with 1 μm-thick sputter-deposited aluminum before assembly for greater internal reflection.
We report a novel switching method that occurs due to the electro-optic effect under applied field when a beam incident on an interface between anti-parallel domains in a sample of LiNbO3 subtends an angle greater than that required for total internal reflection (TIR).
The z confinement of the illumination after passing through these nanoscale apertures was 10 20 times smaller than that for total internal reflection fluorescence (TIRF) microscopy.
Flow chambers for total internal reflection fluorescence (TIRF) microscopy were assembled using biotin-PEG cover glasses from MicroSurfaces, Inc. (Englewood, NJ) or prepared as described (Bieling et al., 2010).
For total internal reflection fluorescennce (TIRF) microscopy studies, Huh7 cells were transfected with pEGFP-C1-AhR or pEGFP-C1 as a control using LT1 transfection reagent (Mirus, Madison, WI, USA).
Some of these angles are beyond the critical angle for total internal reflection and the diffracted light is trapped inside the active film, thus ensuring absorption (see Section 2.1).
Briefly, a multi-line argon krypton laser (Innova70C, Coherent, Santa Clara, CA) and a 405 nm diode laser (Cube, Coherent) were coupled into an inverted microscope (IX71, Olympus) equipped with a 63× oil immersion objective (PlanApo 63×, NA 1.45, Olympus) suitable for total internal reflection fluorescence (TIRF) imaging.
