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The fabricated chip is a monochrome imager including a 256 × 256 pixel array, a complete read-out channel, a series of DACs for internal references and digital blocks for chip control.
The fabricated chip includes a 320×240 pixel array, a complete read-out channel, a 10-bit ADC converter, a series of DACs for internal references and digital blocks for chip control.
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The residual solvent signal at 2.49 ppm for proton and 39.5 ppm for carbon was used for internal referencing of chemical shifts.
For example, ℙ(C1) > 0.7 for internal reference panels of n1 ≥ 400 haplotypes even when considering an extremely large external panel of n2 = 10,000 haplotypes (light blue curve).
Two recent studies have proposed maximizing phylogenetic diversity (PD) as a criterion for internal reference panel selection (Kang and Marjoram 2012; Zhang et al. 2013).
Similarly, no significant drift in assay performance was observed as measured by monitoring peak areas as well peak area CVs for internal reference peptides.
The spectra of the isolated lignins were recorded in DMSO-d6 at 300 K on a Varian Inova 500 MHz spectrometer (Agilent Technologies, Santa Clara, CA, USA) operating at 499.86 MHz for H and 125.7 MHz for C. The residual solvent signal at 2.49 ppm for protons and 39.5 ppm for carbon was used for internal referencing of chemical shifts.
Relative mRNA expression levels were calculated by the comparative cycle threshold (CT) method, using the CT values obtained for TBP as internal references.
We conclude that empirical modeling based on an abiotic stressor gradient can provide an alternative approach for deriving internal reference expectations for great rivers with few, if any, minimally disturbed sites.
GUSB, UBC and SDHA were selected as endogenous controls for real-time RT PCR quantification and used as internal references for normalisation.
We designed five primer pairs for qPCR amplification, and two primer pairs for our internal reference (supplementary table S5, Supplementary Material online).
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