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For internal control, GAPDH was used.
The kit also includes a primer pair for internal control.
For internal control, GAPDH was used as the housekeeping gene.
beta-actin primer was used for internal control.
DNA sequence used for internal control of the qPCR.
For internal control 18S, 30 PCR cycles were used.
Internal quality control was conducted using standard laboratory protocol and procedures, including having slides randomly read by different laboratory technicians or the chief technician/supervisor for internal quality control measures.
Interassay variability, as measured by the average CV for internal controls samples, was between 7 and 9%.
This allows for internal controlling of potential confounders.
Lead concentrations in unprocessed muscle tissue collected from the shank and well away from the bullet path of the same 13 deer were all below the detection limit of 2.0 µg/g and served as internal controls for measures of lead in ground meat.
Internal quality control measures are used for assays.
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