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The optimal expression conditions were detected with 0.1 mM IPTG, 20 °C for induction temperature and induction time for 16 h in our research.
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Additional file 2: Optimization of induction temperature for PschSOD recombinant expression.
In present time both habitats show low temperatures in the winter (e.g. <6°C in the Black Sea), while the only migratory pathway, the Mediterranean Sea, is an isolating barrier because its lowest water temperature is several degrees °C above the strobilation induction temperature for BOR – and probably for LIM-MKL – individuals.
Hence, the optimum induction temperature for α-pinene production was 30°C.
Using face centered central composite design (FCCCD), the optimum induction conditions for high bromelain activity were induction temperature (27°C), L-arabinose concentration (0.15% w/v) and post-induction period of 8 hr.
The recombinant plasmid or the void pET3a vector (control) were introduced into E. coli BL21 (DE3) pLysE cells, and expression of soluble acyltransferase was first tested at standard induction temperature (37°C for 8 h).
In contrast to previous studies (Kim et al. 2007; Zhang et al. 2014) we found that maintaining induction temperature at 37 °C for 20 h led to a low aggregation level of the VEGF165 improving its solubility.
The quantitative analysis of embryo germination (Table 1) showed that slow air desiccation resulted in more than three times higher (50.3 ± 19.8%) embryo germination percentage compared to direct culture of embryos (14.0 ± 4.7%), irrespective of the embryo induction temperature and conditions for incubation.
The optimal medium and several important fermentation conditions (induction time, induction temperature, and initial pH) for SrtA production were obtained by single factor optimization in 250-mL shaking flasks containing 25 mL of sterilized medium.
The probable reason for that might be caused by the induction temperature of 37°C.
For induction, the temperature was reduced to 25°C and the feed was switched to 100% methanol with 12 mL/L PTM1 trace salts at an initial feed rate of 0.6 mL/h until the culture was fully adapted to methanol.
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