Exact(3)
Adriamycin was added to cultures at 0.2 μg/ml (Sigma) for indicated durations.
Cell growth was monitored hourly for indicated durations via calculation of "cell index" (normalized impedance) for each well.
Mice received OVA-containing diets one week after the second injection for indicated durations.
Similar(57)
Neurons were washed and then stimulated with 0 or 25 µM NMDA/TTX for 3 min, and then washed and further incubated for the indicated durations.
All pretreatments, bacteria and bacterial toxins were incubated with epithelial cells at 37°C and 5% CO2 for the indicated durations.
The next subsections will describe neural equivalents of these sub-processes and provide supporting evidence for the indicated durations.
Wild type cells were challenged for the indicated durations prior to survival and transcriptome analyses (Methods).
Hela cells were grown in 60 mm Petri dishes until 80% confluent, and treated with LY30 for the indicated durations.
Flag‐PLK2 and Flag‐TAp73 were purified and were then incubated together in kinase buffer for the indicated durations.
In some experiments cells were treated with recombinant IGF-1 (Invitrogen) or AG538 (EMD Chemical), an IGF-1R selective inhibitor, for the indicated durations.
Proliferating cells in animals were labeled by intraperitoneal injections of BrdU (100 mg/kg body weight, once daily) for the indicated durations.
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