Were the particular domesticates subjected to selection pressures for increased lifespan or allocation to seed yield simply unable to respond?
Genetic data have consistently shown that autophagy is required for increased lifespan in a variety of genetic models [ 44].
However, the level of autophagic activity changes as animals age, and autophagy genes are required for increased lifespan in many genetic long-lived models [ 44, 45].
We noticed continuous exposure to H2S is not required for the effects on proteostasis, although it is for increased lifespan [Fig. 6B,C (Miller & Roth, 2007)].
Additionally, although this new model requires NHR-8 for increased lifespan, eat-2 mutant worms are not affected by nhr-8 knock-down.
However, we found that protective effects of H2S are independent of sir-2.1, which is required for increased lifespan in H2S (Miller & Roth, 2007).
Translocation of DAF-16 to the nucleus and subsequent activation is required for increased lifespan due to reduced IIS, and many DAF-16 target genes have been identified as modulators of lifespan (Lin et al., 1997; Ogg et al., 1997).
Genetic analysis showed that daf-15 likely acts downstream of daf-16 for dauer arrest and daf-16 activity was dispensible for increased lifespan in CeTOR/let363 adults (Fig. 1C) (Vellai et al., 2003).
