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Nonetheless the same tendency for improved expression in hydrogels can be seen as for collagen type II mRNA expression.
Note that wt PTE was obtained in previous screens for improved expression levels in E. coli and contains six mutations relative to the naturally occurring PTE (I106L, F132L, K185R, D208G, R319S) (Roodveldt and Tawfik, 2005 ; Tokuriki et al., 2012 ).
The CXCR2 peptide was fused to the N-terminus of the B1 domain of protein G (GB1) for improved expression (Huth et al, 1997) and provided with a C-terminal histidine (His) tag to allow purification.
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A main prerequisite for a successful epMEGAWHOP experiments is a reliable high-throughput screening system which allows an identification of improved expression variants.
A possible explanation for the improved expression charateristics of "dkr" compared to the complete ORF may be indicated by codon usage analysis: Compared to L. lactis subsp. cremoris MG1363 and Lb.
Starting from a model for damaged suspended cables recently proposed in the literature, an improved expression for the dynamic increment of cable tension is derived.
For a thick shell under external hydrostatic pressure, the stability equations are solved analytically and yield an improved expression for the buckling load.
The carried out accurate measurements for four different kinds of surfaces of the experimental model allow a fine-tuning of the improved expression for convection heat transfer coefficient hc.
Then, an improved expression for the damage growth and accumulation under fatigue loading was adopted (Van Paepegem and Degrieck 2001): frac{dD}{dn}=left{begin{array}{c}frac{A{left(frac{varDelta sigma}{sigma_{TS}}right)}^c}{{left 1-Dright)}^b}kern4em in tension, 0kern4em in compression,end{left 1-Drightern0.75em (55).
Moreover, promoter and 3' UTR regions of genes [ 13] can now be used to design vectors for D. salina with improved expression levels.
Biocatalysis has widened its scope and relevance since new molecular tools, including improved expression systems for proteins, protein and metabolic engineering, and rational techniques for immobilization, have become available.
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