Sentence examples for for immunoprecipitation experiments from inspiring English sources

For immunoprecipitation experiments, 0.5 - 1 mg of proteins were immunoprecipitated with the appropriate antibody for 1 h at 4°C in the presence of 25 μl of protein A/G agarose (Amersham).

Beads were then washed 5 times with IP lysis buffer and immunoprecipitates were eluted in Laemmli buffer at 99 °C for 10 min. For immunoprecipitation experiments of mitochondrial and cytosolic fractions, the cells were fractionated in advance as described below.

COS7 cells were utilized for immunoprecipitation experiments.

Anti-RPB4 antibody, produced in rabbits from recombinant protein as antigen, was used for immunoprecipitation experiments.

For immunoprecipitation experiments, the lysis buffer contained 0.3% CHAPS instead of 1% Triton in order to preserve the integrity of the mTOR complexes.

To prepare cells for immunoprecipitation experiments, 50 ml of cells at OD600 0.7 were resuspended in YPD containing 30 µg/ml benomyl, followed by growth at room temperature for 3 hours.

For immunoprecipitation experiment, the total protein lysate was used to immunoprecipitate ubiquitin or ubiquitinated proteins using the mouse monoclonal Ub antibody (1 µg, scbt) and protein A/G agarose beads (scbt) as described before [40].

Cells were lysed and then incubated with anti-FasAPO-1-1 and protein G agarose for immunoprecipitation experiment.

We used antibodies for total c-met and Stat3 for reciprocal immunoprecipitation experiments with WT and DN2 cells treated with HGF.

Cells were washed with PBS, then cross-linked in vivo with 1% formaldehyde in PBS for 10 min at 37°C, as for chromatin immunoprecipitation experiments (Solomon et al, 1988).

Then the cells were harvested, sorted into cell cycle stages and used for chromatin immunoprecipitation experiments.