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For immunohistochemical methods see Supplementary File.
All lung samples were stained for standard histopathological analysis and for immunohistochemical methods using a specific mouse monoclonal antibody.
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Furthermore, the biochemical methods have been abandoned to the detriment of immunohistochemical methods for hormone receptor assessment in an increasing number of pathology laboratories.
6– 8 Great advances have been made in TAM research over the past dozen years or so, with one of the most significant breakthroughs being the development of immunohistochemical methods for identifying TAMs in tumor tissue.
This approach is especially important for standardizing immunohistochemical methods across labs and for attempting to multiplex IHC in fixed tissue.
CH planned the study, was responsible for the immunohistochemical methods, and helped to draft the manuscript.
We have previously performed several studies on MPT64 for the development of immunohistochemical method using patient biopsies and aspirate samples to diagnose extrapulmonary TB [ 21– 21].
Materials for biochemical and immunohistochemical methods are listed in the appropriate sections.
In order to analyze whether brachyury may be also used for the differentiation of renal HBL from adrenal carcinoma or paraganglioma, we tested the expression of this marker in five adrenal carcinomas as well as in five paraganglioma (unpublished data) (see [ 26] for details on immunohistochemical methods).
For example, when we use two fecal occult blood tests (occult blood tests and immunohistochemical methods) for colon cancer screening, a positive occult blood test is often accompanied by a positive immunochemical result; thus, the results of the two tests are dependent [ 2].
Immunohistochemical methods for the detection of surface markers are relatively insensitive and difficult to standardise.
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