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Samples were stored on ice for immediate analyses or frozen at −80°C for further analyses.
The samples will be transported to the BioBank for immediate analyses to generate the hematology profile using the laboratory's Coulter analyzer.
Twelve fruit of each maturity stage (medium size, M; mature green, MG; breaker, BR; light orange, LO; orange, O; pink, P; red, R and red ripe, RR) were harvested for immediate analyses of fruit color, firmness, ethylene production and respiration rate (CO2 production) as described by [ 22, 50].
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In this study, the tests were performed according to recently published guidelines concerning devices for breath sampling, stationary and immediate analyses, prolonged expiration and correction for alveolar CO2, use of antibiotics, diet, cigarette smoking and physical exercise [ 15].
Compared with conventional injections of ESCs into blastocyst host, eight cell-stage embryo injection efficiently yields F0 generation mice, allowing for immediate phenotypic analyses (Poueymirou et al., 2007).
Previously, it was reported that laser-assisted microinjection of ES cells into an eight-cell-stage diploid embryo efficiently provides founder generation mice that are available for immediate phenotypic analyses (Poueymirou et al., 2007).
Cells were washed twice and resuspended in FACS buffer containing 1 µg/ml Actinomycin D. After 5 min incubation, all samples were fixed in 500 µl 1% EM grade formaldehyde (Polysciences, Warrington, PA) for immediate flow cytometry analyses.
Aliquots were sent to the biochemistry and microbiology laboratories for immediate processing and analyses.
The carotid and renal venous blood samples were used for immediate arterial blood gas analyses performed on an ABL 700 (Radiometer, Copenhagen, Denmark).
Twenty-day-old calli were harvested and used for immediate cellular and biochemical analyses or stored at -80°C for later molecular analysis.
Fifteen-day-old calli were harvested and used for immediate cellular and biochemical analyses or stored at -80°C for molecular analysis.
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