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In a first step, a pairwise alignment of these sequences was carried out using the needle algorithm of emboss suite (http://www.ebi.ac.uk/emboss/align/index.html), thus allowing for identity determination between the 12 RickA proteins.
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This situation had significant negative economic consequences for the region and motivates continued efforts, such as the molecular and statistical methods covered here, to better quantify accuracy for individual‐based population identity determination for improved management, monitoring and conservation.
At least one of the redundant SEP proteins is required for floral organ identity determination where specificity is determined through the combination of A, B and C function proteins [ 3].
The criteria for a successful identity determination were: at least 3 matched peptides and 20% sequence coverage; isoelectric point differing by at most 2; and molecular weight differing by at most 20% compared with the experimental estimate.
As the SEP-like Gerbera gene GRCD1 is needed for stamen (more precisely, staminode) identity determination, and GRCD2 for carpel identity determination, we have concluded that the homeotic E function has evolved into a whorl-specific set of subfunctionalized gene paralogs [ 41, 42].
This not only suggests that the mechanisms of organ identity determination for second whorl organs were established early during the evolution of the angiosperms, but also that the subsequent morphological divergence in the grasses is probably associated with the lineage-specific substitutions in the K-domain that we detected.
The role of mechanical signals in cell identity determination remains poorly explored in tissues.
Previous studies on floret mutants only provided a superficial understanding of floral organ identity determination in wheat plants.
STK also acts redundantly with SHP1 and SHP2 to promote ovule identity determination (Colombo et al., 2010).
Previous research has indicated that members in AP2-EREBP gene family play a role in floral organ identity determination [ 27].
Who is responsible for identity?
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